The osmotic potential of a model cell  Essay Assignment Paper

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 The osmotic potential of a model cell  Essay Assignment Paper

 

 

You design an experiment to test the effect(s) various compounds have on the osmotic potential of a model cell. You know that substances dissolved in aqueous or gaseous solutions tend to diffuse from regions of higher concentration to regions of lower concentration. You fill each of three (20mL) dialysis bags half full with one of these substances: i. 5% by weight of glucose in distilled water ii. 5% by weight of egg albumin (protein) in distilled water iii. 5% by weight of glass bead (one glass bead) in distilled water The dialysis bag is permeable to water but impermeable to glucose, albumin, and glass bead. a) If the final weight of each prepared bag is 10g, how many grams of glucose, albumin, and glass bead were added to each bag? b) The molecular weight of the protein is about 45 kilodaltons, and the molecular weight of glucose is about 180 daltons. How can you estimate the number of molecules of glucose in its 5% solution compared to the number of albumin molecules in its 5% solution? c) You put the dialysis bags into three separate flasks filled with distilled water. After 2 hours, you remove the bags and record these weights: Dialysis Bag Weight Glucose 13.2g Albumin 10.1g Glass bead 10.0g How do you explain these results? (hint: consider the surface-area-to-volume ratio, or concentration, of each substance based on its molecular weight) d) What results would you predict if you set up a similar experiment but used 5% glucose and 5% sucrose (MW 342 daltons)? II. How is the structure of a cell membrane related to its function? 1. Substances can move across the membrane via simple diffusion, facilitated diffusion, or active transport. Fill in the table below with information about each process. a) Where does it occur in the membrane? b) Does it require a transport protein? c) Does it require an input of energy? Simple Diffusion Facilitated Diffusion Active Transport d) What functions might each of the three types of diffusion serve in an independent cell, such as Paramecium or an amoeba? e) What functions might each of the three types of diffusion serve in a multicellular organism, such as a human or a tree? 2. What would you need to observe or measure to determine whether a substance was moved across a membrane via each type of diffusion? Fill in the table below. Simple Diffusion Facilitated Diffusion Active Transport

 

 

 

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